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Incidence of Cymbidium mosaic virus (CymMV) in Sikkim.

机译:锡金花兰花叶病毒(CymMV)的发病率。

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This study analysed the incidence of Cymbidium mosaic virus (CymMV) in orchids from Sikkim, India. One hundred plants comprising species and hybrids belonging to 24 orchid genera from Sikkim were screened for the presence of CymMV using ELISA, RT-PCR and Northern slot blot hybridization techniques. Results indicated the widespread occurrence of CymMV in the orchids tested. Orchid samples which were symptomless and gave negative result in ELISA were found to be positive in RT-PCR and Northern slot blot.
机译:这项研究分析了来自印度锡金的兰花中大花mosaic兰花叶病毒(CymMV)的发生率。使用ELISA,RT-PCR和Northern狭缝印迹杂交技术,筛选了来自Sikkim的属于24个兰花属的物种和杂种的一百株植物中是否存在CymMV。结果表明CymMV在所测试的兰花中广泛发生。在RT-PCR和Northern印迹法中发现无症状并在ELISA中产生阴性结果的兰花样品为阳性。

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