Effects of 2.45‐GHz microwave and 100‐MHz radiofrequency radiation on liposome permeability at the phase transition temperature

摘要

AbstractLarge unilamellar dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphos‐phatidylglycerol (DPPG) liposomes loaded with an aqueous chemotherapeutic drug, cytosine arabinofuranoside (ARA‐C), were exposed for 30 min to 60 W/kg continuous‐wave (CW) 100‐MHz or 2.45‐GHz radiation in vitro at temperatures between 37 °C and 43 °C. Liposomes were exposed in HEPES buffer or in HEPES buffer supplemented with 44 by volume fetal calf serum (FCS). Characteristic phase transition responses were detected in the range of 39 °C to 40 °C with the presence of FCS, increasing maximum release of3H‐ARA‐C by 20 relative to HEPES suspension. Neither frequency of electromagnetic radiation had any detectable effect on liposome permeability or the location of the phase transition in the presence