AbstractThe acute toxicity of copper and copper plus complexing agents to common guppyLebistes reticulatuswas studied for 96 h by a static bioassay technique. The addition of complexing agents viz. disodium salt of EDTA, citric acid, sodium thiosulphate and glycine in Cu2+solutions caused a great decrease in the per‐cent mortality as compared to that of Cu2+test solutions alone. 10 mg/l of complexing agent was added in each copper concentration in all the test series. The 96 h LC50values and 95 confidence limits in mg/l of Cu2+plus were 1.23 (0.95…1.65) for Cu2+alone; 4.30 (4.04 … 4.55) for Cu2+plus EDTA; 1.94 (1.69 … 2.18) for Cu2+plus citric acid; 3.44 (2.96 … 3.74) for Cu2+sodium thiosulphate and 2.29 (2.22 … 3.02) for Cu2+p
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