Molecular identification of a new strain Narcissus yellow stripe virus associated with severe mosaic disease of Narcissus from India

摘要

A Potyvirus associated with severe mosaic disease of Narcissus tazetta in India was characterized at molecular level. Nucleotide sequence of its 3-terminal genome (-2900 bp) comprising 3 -non translated region (NTR), large nuclear inclusion protein (Nib, RdRp, replicase), coat protein (CP) and C-terminus of Nla-proteinase (Nla-Pro) showed 93% and 85% homology whereas, CP gene showed 97% and 87% identities with Narcissus yellow stripe virus (NYSV) at amino acid and nucleotide level, respectively. The3-NTR based sequence comparison showed maximum homology of 98% with NYSV. Phylogenetic analyses of present viral genome showed its close relatedness to NYSV, at nucleotide and amino acid level using CP, Nib and 3-NTR sequences. Thus, it may be concludedthat it is a new strain of Narcissus yellow stripe virusirom India.