Proliferation Kinetics of Rat Kupffer Cells after Partial Hepatectomy Immunohistochemical and UItrastructural Analysis

摘要

In an effort to settle the conflicting views on the proliferation kinetics of Kupffer cells (Kc), we performed 2/3 partial hepatectomy on rats injected with Pelikan ink. Using an anti‐rat macrophage monoclonal antibody, ED 2, we evaluated the numerical changes in total, carbon‐positive ED 2+cells and carbon‐negative ED 2+cells in the portal and central area. We also analyzed the ultrastructure and peroxidase cytochemistry of various types of cells observed during regeneration. The total numbers of ED 2+cells in the remaining liver increased rapidly from day 2 to 5, and the number of dividing ED 2+cells reached a maximum on day 2. Thus, the numerical increase in ED 2+cells corresponded to the division phase. In contrast, the carbon‐labeling experiment showed a continuous increase of carbon negative ED 2+cells from day 2 to 7. In the central area where division was less frequent, the proportion of carbon‐positive cells decreased markedly to 50 on day 7, as against 97 in control rats. These findings suggest the possibility of an influx of carbon‐negative Kc in addition to cell division. Ultrastructurally, the presence of carbon‐negative “small Kc” and “immature Kc” with morphological features different from those of carbon‐positive Kc was demonstrated. Such carbon‐negative Kc with a high nucleus‐to‐cytoplasm ratio and rather few phagosomes, were not observed in control rats. Furthermore, we demonstrated two types of possible precursor cell, i.e. “transitional” forms between monocytes and Kc, and “immature macrophages”. The former showed peroxidase activity in some lysosomes as well as in the rough endoplasmic reticulum and nuclear envelope. Our result indicated that the proliferation kinetics of Kc depend upo