AbstractRecombinant cytochrome bs was extracted to a reversed micelle phase of a cationic surfactant (hexadecyltrimethylammonium bromide, CTAB) in cyclohexane/decanol and back‐extracted to a fresh aqueous phase. Depending on the experimental conditions, i.e., temperature, pH, and ionic strength, the extraction is controlled by either hydrophobic (pH close to the pIand high ionic strength) or electrostatic (pH far from the pIand low ionic strength) interactions. The protein interacts strongly with the surfactant aggregate once it is extracted to the organic phase, and it is very difficult to back‐extract it to a new aqueous ph
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机译:摘要将阳离子表面活性剂(十六烷基三甲基溴化铵,CTAB)在环己烷/癸醇溶液中提取重组细胞色素bs,并反向提取至新鲜水相。根据实验条件,即温度、pH 值和离子强度,萃取由疏水(pH 值接近 pI 和高离子强度)或静电(pH 值远离 pI和低离子强度)相互作用控制。一旦蛋白质被提取到有机相,蛋白质就会与表面活性剂聚集体发生强烈的相互作用,并且很难将其反提取到新的水性pH值
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