Glycoprotein Associated with Funneliformis coronatum , Gigaspora margarita and Acaulospora scrobiculata Suppress the Plant Pathogens In vitro

摘要

Background and Objective: Arbuscular mycorrhizal fungi (AMF) form symbiotic association with most plants and are known to play significant role in higher nutrient uptake and soil aggregation as well as, carbon sequestration through production of specific glycoprotein characterized as glomalin or glomalin related soil protein (GRSP) contained in their hyphae and spores. The objective of this study was to find out the fungi static effects of this glycoprotein in suppression of growth of two soil borne plant pathogens in vitro . Materials and Methods: Sorghum was used as the test plant, Funneliformis coronatum ( F. coronatum ), Acaulospora scrobiculata ( A . scrobiculata ) and Gigaspora margarita ( G. margarita ) were used as AMF inoculums, Rhizoctonia solani ( R . solani ) and Colletotrichum falcatum ( C. falcatum ) were used as test pathogens. The GRSP was extracted from the soil of sorghum pots after harvesting, two different concentrations of GRSP was supplied to the agar medium and test pathogens were inoculated, control plates received extractant from non-AMF inoculated soil. The experiment was performed in completely randomized design with 4 replicates each. The data were analyzed by one-way ANOVA. Results: The level of GRSP production varied with different AMF species. In vitro testing of suppression of Rhizoctonia solani and Colletotrichum falcatum by GRSP extracted from Funneliformis coronatum inoculated soil was higher followed by Gigaspora margarita and Acaulospora scrobiculata inoculated soil. Conclusion: Production of GRSP differs with AMF species and was found to suppress the growth of pathogens in vitro .