Quantitative Bestimmung primärer aliphatischer Alkohole (C6-C10) mit Hilfe einer pyridinnukleotidunabhängigen Alkoholdehydrogenase ausPseudomonas putida

摘要

A new enzymatic method for microdetermination of longer chain alcohols has been established withNAD(P)-independent alcohol dehydrogenase from an alkane-oxidizing strain ofPseudomonas putida. The alcohols were estimated spectrophotometrically by measuring the formazan formation from the corresponding tetrazolium salt. Amounts of longer chain alcohols up to 3 nmol could be assayed by reading the endpoint of the reaction, even in presence of a 1000-fold exess of ethanol.