Rapid analysis of glufosinate by improving the bulletin method and its application to soybean and corn Japanese

摘要

A rapid analytical method for residues of the herbicide glufosinate DL-homoalanin-4-yl (methyl)phosphinic acid and its metabolite (MPPA:3-methylphosphinicopropionic acid) in soybeans and corns was developed by improving the bulletin method. Fifty mL of solution extracted with water (corresponding to 2 g of the sample) was loaded on a column packed with 5 mL of anion exchange resin, and then the trapped glufosinate and MPPA were eluted with 40 mL of 50 acetic acid. After the derivatization of glufosinate and MPPA with trimethyl orthoacetate, the derivatives were purified and separated on a silica gel cartridge column. The determination of the derivatives was performed with a GC-FPD. The detection limits for glufosinate and MPPA were 0.01 mug/g and 0.005 mug/g, respectively. The recoveries of glufosinate from soybeans and corns were 86.3similar to92.0, and those of MPPA were 86.5similar to95.2.