Characterization of Antibodies Against Human N-Terminal Parathyroid Hormone by Epitope Mapping

摘要

Two polyclonal antisera from goat and mouse and two monoclonal antibodies against human parathyroid hormone (1#x2013;34) were characterised by epitope mapping. Hexapeptides were synthesized on polystyrene pins, the sequences of which overlapped and represented the entire sequence of hPTH(1#x2013;34). Binding of antibodies to these hexapeptides was determined and antigenic determinants thus characterized. At least one predominant binding sequence was detected in the region of hPTH(7#x2013;14).