Common and specific antigens of several treponemes detected by polyclonal antisera against major cellular proteins

摘要

Thirteen polypeptide antigens with molecular weights ranging from 34 kDa to 83 kDa were selected and their antigenic behaviors and distribution were examined in 12 strains of microorganisms includingTreponema, Borrelia, Leptospira and Leptonema.Immunoblot analysis demonstrated that 45 kDa and 83 kDa polypeptides ofTreponema socranskiisubsp.buccaleATCC 35534, 53 kDa antigen of Treponema denticola ATCC 33520 and 44 kDa polypeptide of the strain G7201 were strain‐specific. The 34, 62, 66 and 84 kDa polypeptide antigens were detected in all 8 treponemal strains examined.T. denticolaATCC 33520 and ATCC 35404 possessed 38 kDa, 48 kDa, 52 kDa and 72 kDa common polypeptide antigens. All 12 strains possessed the 84 kDa polypeptide antigen. Immunoelectron microscopy demonstrated that the 34 kDa and 38 kDa polypeptide antigens were located on the axial flagella and that other polypeptide antigens were located on the outer envelopes or wall‐membrane comple