Syngeneic sensitization of mouse lymphocytes on monolayers of thyroid epithelial Cells. II. T and B cell involvement in primary responses

摘要

AbstractUsing a previously developedin vitromodel of sensitization of lymphoid cells against monolayers of syngeneic thyroid epithelial cells, the T or B nature of the population involved in the primary response has been investigated. For this purpose normal good responder CBA strain mice were used. T cell participation was clearly demonstrated in two ways: first, by the loss of primary sensitization after T cell depletion accomplished using several different techniques (includingin vivoandin vitroanti‐Thy‐1.2 treatment, hydrocortisone and cyclophosphamide injections) and second, by the high response (measured by tritiated thymidine uptake) of T cell‐enriched suspensions obtained either by nylon wool filtration or Ig‐anti‐I