Clonal propagation of mature trees of Gmelina arborea Roxb. via in-vitro technique.

摘要

In-vitro axillary bud proliferation has been achieved in nodal segment explants from the side branches of 15-18 years old trees of Gmelina arborea on MS medium +10 micro M BA. MS medium containing 1.0 micro M BA and 0.1 micro M Kn induced maximum shoot multiplication. The addition of 23 micro M AgNO₃ in the culture facilitated callus free shoot formation. Rooting was achieved on 1/2 MS medium enriched with 10.0 micro M IBA and rooted plantlets were successfully acclimatized in high humidity conditions (80-90% RH) for two weeks prior to successful transfer to a shadehouse. The eight months old potted plants were showing excellent growth and development.