Leucoagaricus leucothites (Vittad.) Wasser is an edible macrofungus found limitedly in Turkey. The ethanolic extract of L. leucothites has shown strong antimicrobial activity against some foodborne and spoilage bacteria. Therefore, the phenolic composition of L. leucothites ethanolic extract was analyzed by using high-performance liquid chromatography. Catechin (288 mg/L) was found to be the major component in the extract. Antioxidant activity of the extract was evaluated by studying the scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, and chelating ability of the extract was assessed in a ferrous ion assay. The scavenging effect on DPPH radicals was 71 at 10 mg/mL, whereas the chelating effect on ferrous ions was 82 at 15 mg/mL. The total contents of phenol (3.40 +/- 0.5 mg/g), ascorbic acid (2.10 +/- 0.3 mg/g), beta-carotene (0.02 +/- 0.0 mg/g), and lycopene (0.01 +/- 0.0 mg/g) in the macrofungi ethanolic extract were also determined.
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