Resolution in column chromatography of polymer latexes. I. The light scattering behavior of polystyrene and its effect on signal resolution in HDC

摘要

AbstractA method for improving signal resolution in latex particle‐size analysis by HDC is presented and discussed. Data for the extinction cross section and specific extinction coefficient for polystyrene latex standards indicate that improvements in signal resolution can be obtained for the small particle end of broad size distributions by using turbidity detection at wavelengths less than 254 nm. Data are also discussed for the imaginary refractive index of the polystyrene standards obtained from comparisons of the extinction cross section results with Mie theory calculations. HDC runs made with bimodal and continuous distributions at 254 and 220 nm wavelength detection are also discussed to illustrate the improvements in size distribution resolution which can resul