In the present study it was found that uraemic plasma contains inhibitors which affect insulin binding on erythrocyte insulin receptors. Insulin radioreceptor assays performed in the absence of uraemic plasma revealed no defects in the receptors of the uraemic erythrocytes. Uraemic plasma caused a 48decrease in insulin binding on healthy erythrocytes at a high (10.1 ng/ml) insulin concentration, but only a 17decrease at a low (70 pg/ml) insulin concentration. The inhibition of insulin binding by uraemic plasma decreased during a haemodialysis treatment from 18to 13at the low insulin concentration, and from 30to 23at the high insulin concentration. Insulin insensitivity in uraemia appears to be due to inhibition of the insulin receptors.
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