DEVELOPMENT OF ULTRAVIOLET SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF BRIMONIDINE TARTRATE AND BRINZOLAMIDE

摘要

A simple, accurate and precise UV spectrophotometric method has been developed for the simultaneous estimation of brimonidine tartrate (BRT) and brinzolamide (BRZ) using methanol as solvent. The for BRT and BRZ were found to be 244 nm and 253 nm, respectively. The linearity was found in the range of 1-7 ug/mL and 5- 35 ug/mL for BRT and BRZ, respectively. The LOD and LOQ for BRT were found to be 0.0149 ug/mL and 0.0425 ug/mL at 244 nm and 0.0278 ug/mL and 0.0843 ug/mL at 253 nm, respectively whereas those for BRZ were found to be 0.0330 ug/mL and 0.1000 ug/mL at 244 nm and 0.0243 ug/mL and 0.0737 ug/mL at 253 nm, respectively. The % recovery of the drugs by the proposed method was found in the range of 98.29% to 100.82%. The method was found to be precise and robust. The method was successfully applied for determination of BRT and BRZ in an in-house suspension.