A SIMPLE BIOPROCESSING METHOD FOR COLLAGEN PEPTIDE FROM THE FISH OFFALS OF RASTRELLIGER KANAGURTA AND ITS HPTLC QUANTITATION

摘要

The present study describes the isolation and extraction of collagen and collagen peptide from the crude, fish offals of the common Indian macreal fish {Rastrelliger kanagurta), which makes the total extraction process more economical compared to the existing industrial procedures. Hydrolyzed collagen provides additional amounts of amino acids like glycine, proline, and hydroxy proline, which are specially needed for the body to support connective tissue structure and to maintain healthy cell function. These three amino acids are present at a high concentration in collagen peptide compared to other amino acids. The yield of collagen peptide was found to be 34% w/w. The HPTLC analysis of RFCP (Rastrelliger kanagurta Collagen Peptide) and the marketed collagen peptide sample revealed that the amount of proline present is 2.33 and 0.86% w/w respectively. The amino acid glycine was not detected in the marketed sample but the laboratory extracted sample (RFCP) showed 0.26% w/w.