A standard protocol for rapid propagation ofAsparagus robustusHort. from callus has been developed. Callusing and regeneration were maximum using segments of shoot tissue. Shoot and internode tissue retained the ability to form callus with high regenerability in culture for 34 months. Basal medium supplemented with 3 mg/liter 2,4-D and 1 mg/liter Kn were most effective in formation of callus. Shoot formation was optimum on medium containing 0.1 mg/liter NAA, 1 mg/liter BAP, and 40 mg/liter adenine sulfate. Root induction was maximized in half-strength MS basal medium with 0.5 mg/liter IBA. The number and length of roots was greater under an 8 h photoperiod as compared with a 16 h photoperiod. Regenerated plants could be maintained in half-strength MS liquid medium for 30 days prior to transfer to potted soil.
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