Isolated goat ileum preparation: An alternative to isolated ileum preparation from laboratory animals

摘要

Isolated tissue preparations are commonly used to study the effects of drugs on specific type of receptors. These preparations are also used for bioassay of drugs, characterisation of specific receptor or its subtypes, to determine concentration response curve of an agonist, to study antagonism of drug and in new drug discovery. Isolated heart and rectus abdominis of frog are the two commonly used preparations to teach undergraduate (UG) and postgraduate (PG) students.The Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), has instructed universities and institutions not to use frog for teaching purposes, as there is no licensed breeder of frog in India. This has driven us to conduct alternative isolated tissue experiments.