Simultaneous Determination of Clobazamcomma; Nhyphen;Desmethyl Clobazam and Clonazepam in Plasma by High Performance Liquid Chromatography

摘要

A method is described for the simultaneous determination of clobazam, its active metabolite /V-desmethylclobazam, and clonazepam in plasma by high performance liquid chromatography. The drugs are extracted with diethyl ether from 1.0 ml plasma adjusted to pH 9.0 and separated on a C18reverse phase column using a mobile phase of 40percnt; acetonitrile in phosphate buffer pH 3.0. The ultraviolet absorbance is monitored at a wavelength of 313 nm. The method has a coefficient of variation of 5percnt; and an overall recovery of 85percnt; and is sufficiently sensitive for the therapeutic monitoring of these drugs.