Integrative Approach for Producing Hydrogen and Polyhydroxyalkanoate from Mixed Wastes of Biological Origin

摘要

In this study, an integrative approach to produce biohydrogen (H-2) and polyhydroxyalkanoates (PHA) from the wastes of biological origin was investigated. A defined set of mixed cultures was used for hydrolysis and the hydrolysates were used to produce H-2. The effluent from H-2 production stage was used for PHA production. Under batch culture, a maximum of 62 l H-2/kg of pure potato peels (Total solid, TS 2 %, w/v) and 54 l H-2/kg of mixed biowastes (MBW1) was recorded. Using effluent from the H-2 production stage of biowaste mixture (MBW1), Bacillus cereus EGU43 could produce 195 mg PHA/l and 15.6 % (w/w). Further, supplementation of GM-2 medium (0.1x) and glucose (0.5 %) in H-2 production stage effluents, resulted in significant improvements of up to 11 and 41.7 % of PHA contents, respectively. An improvement of 3.9- and 17-fold in PHA yields as compared to with and without integrative H-2 production from the MBW1 has been recorded. This integrative approach seems to be a suitable process to improve the yields of H-2 and PHA by mixing biowastes.