Applicability of Reversed-Phase Base-Deactivated Columns for Systematic Toxicological Analysis

摘要

The chromatographic properties of seven reversed-phase columns from different manufacturers, specially prepared for analysis of basic drugs, were investigated. Three test mixtures were used: Neutral (1-nitroalkanes), acidic (salicylamide and four barbiturates) and basic (diphenhydramine, imipramine, amitriptyline, fluphenazine and thioridazine). The mixtures were eluted with three mobile phases, consisting of acetonitrile-water, acetoni-trile-phosphate buffer and acetonitrile-triethylammonium phosphate buffer. The concentration of acetonitrile, pH and molarity of buffers were identical.