Photochemically enhanced adenoviral transduction in a multicellular environment

摘要

Photochemical internalization (PCI) enhances adenovirus (Ad) transgene expression in a variety of cell lines in vitro. However, measurements of the photochemical effect on transduction in multicellular environments are lacking. In this study, spheroids of DU 145 prostate cancer cells were used as a model to evaluate Ad serotype 5 (Ad5) transduction in a multicellular environment in response to PCI treatment. Furthermore, the Ad5 was coated with poly(2-methyl-acrylic acid 2-(2-(dimethylamino)-ethyl)-methyl-amino-ethyl ester) (pDAMA) to evaluate whether physicochemical properties such as charge and size of viral vectors affect transcluction of photochemically treated spheroids.