Loss of serological determinants does not affect recognition of H‐2Kktarget cells by an influenza‐specific cytotoxic T cell clone

摘要

AbstractThe recognition of “self determinants” by an H‐2Kk‐restricted cytotoxic T (Tc) cell clone (K5) has been investigated as follows: (a) differential inhibition of cytotoxicity by several monoclonal antibodies directed to determinants on the H‐2Kkmolecule of the target cells and (b) recognition and lysis of target cell variants of cell line LDHB, which have lost the majority or all of the serological determinants defined by the inhibiting antibodies while still expressing an H‐2Kkmolecule. Such variant cells infected with influenza virus were effectively recognized by Tccell clone K5, whereas a target cell line, which lacks the Kkmolecule, was not lysed. The results suggest; (a) that virus‐specific Tccell see “self” in a manner distinct from the recognition of serological determinants by B cell and (b) that antibody inhibition indicates conformational closeness, but not identity, of the class I determinan