Response related enzymatic changes in ovaries of superovulated mice

摘要

Adult female mice were superovulated with PMSG followed by HCG and 140 blastocyste and 69 morulae were recovered from 24 mice. On the basis of the response, mice were divided into six groups; non respondere, 1-5,6-10, 11-20,21-30 and >30 embryos. Theovaries of the animals were pooled group wise, homogenized in PBS (pH 7.4) and after coitrifugation for 10-15 minutes, the supernatant was analyzed for the enzymes, guanine oxaloacelate transaminase (GOT), guanine pyruvate transaminase (GPT), acid phosphatases (ACP) and alkaline phosphalases (AKP). Acid and alkaline phosphatese activities did not show any variation in relation to response to superovulation but GOT and GPT showed significantly increased activity in response to induction of superovulation. A statistically significant positive correlation was found between GOT and GPT activities and the superovulatory response in mice.