Genetic ‘Fingerprinting’ of Sunflower Lines and F1Hybrids using. Isozymes, Simple and Repetitive Sequences as Hybridization Probes, and Random Primers for PCR

摘要

AbstractThree different marker systems which have been widely described in the characterization of genotypes were compared. Sunflower lines and hybrids were analysed by three isozymes, oligonucleotides (GACA)4, (GATA)4, and (GGAT)4as hybridization probes, and 70 primers for PCR analysis. Polymorphisms were visible with each type of marker. However, the repeated probes, especially (GATA)4, revealed the highest degree of variability and seem to be most appropriate for the generation of genotype‐specific ‘fingerprin