In vitro regeneration of roots of Phyla nodiflora and Leptadenia reticulata, and comparison of roots from cultured and natural plants for secondary metabolites

摘要

Adventitious roots, generated using leaf explains of P. nodiflora, and meristem explants of L. reticulata, were cultured on Murashige and Skoog (MS) medium supplemented with napthylacetic acid (2 #mu#M) and indole butyric acid (3 #mu#M) respectively.After 30 days, subculturing of roots in liquid MS medium with napthylacetic acid (1.5 #mu#M) for P. nodiflora and indole butyric acid (3 #mu#M) for L. reticulata afforded considerable increase in root mass. HPTLC profiles and microscopic examination oftransverse sections of in vitro and naturally grown roots provided information on secondary metabolite accumulation vis-a-vis developmental stages of the root.