Isolation, sequencing, and characterization ofcrp-5, a gene encoding aNeurosporaribosomal protein

摘要

ANeurospora crassacytoplasmic ribosomal protein gene,crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolatecrp-5genomic sequences. Computer analysis of the DNA sequences showed that they contain an open reading frame which encodes a protein homologous to the rat ribosomal protein S26. Thecrp-5mRNA levels are regulated in a carbon-source-dependent manner. The organization of the gene and the region upstream of the coding sequences are discussed.