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Increased longevity of hematopoiesis in continuous marrow cultures and radiation resistance of marrow stromal and hematopoietic progenitor cells from caspase-1 homozygous recombinant-negative (knockout) mice

机译:来自caspase-1纯合重组阴性(基因敲除)小鼠的连续骨髓培养中造血功能的寿命延长以及骨髓基质和造血祖细胞的辐射抗性

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Aim: We determined whether absence of caspase-1 altered the stress response of hematopoietic and bone marrow stromal cells in vitro. Materials and Methods: Longterm bone marrow cultures from caspase-1 -/- and control caspase-1 +/+ mice were established and the derived bone marrow stromal and interleukin-3 (Il-3)-dependent hematopoietic progenitor cell lines were evaluated for radiosensitivity. Results: Long-term bone marrow cultures from caspase-1 -/- mice generated hematopoietic cells for over 30 weeks in vitro, significantly longer than controls did (p=0.0018). Bone marrow stromal (mesenchymal stem cell) and Il-3-dependent hematopoietic progenitor cell lines from caspase-1-/- marrow cultures compared to caspase-1 +/+ were radioresistant (p=0.0486 and p=0.0235 respectively). Total-body irradiated caspase-1 -/- mice were not significantly radioresistant compared to controls (p=0.6542). Conclusion: Caspase-1 deletion increases hematopoiesis and radioresistance of bone marrow cells in vitro.
机译:目的:我们确定caspase-1的缺失是否在体外改变了造血和骨髓基质细胞的应激反应。材料和方法:建立来自caspase-1-/-和对照caspase-1 + / +小鼠的长期骨髓培养物,并评估衍生的骨髓基质和白介素3(Il-3)依赖性造血祖细胞系的表达。放射敏感性。结果:来自caspase-1-/-小鼠的长期骨髓培养在体外产生了超过30周的造血细胞,明显长于对照组(p = 0.0018)。与caspase-1 + / +相比,来自caspase-1-/-骨髓培养物的骨髓基质(间充质干细胞)和Il-3依赖性造血祖细胞系具有放射抗性(分别为p = 0.0486和p = 0.0235)。与对照相比,全身照射的caspase-1-/-小鼠没有明显的放射抗性(p = 0.6542)。结论:Caspase-1缺失可增加体外造血和骨髓细胞的放射抗性。

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