In vitro Granulocyte Function as Affected by Filter Loading Time and Tapping during Filtration Leukapheresis

摘要

Abstract.Granulocytes (PMNs) were harvested from 16 normal subjects by filtration leukapheresis (FL) in which the filter loading time (FLT) ranged between 30 min and 4 h, and the rate of filter perfusion was 60 ml whole blood · min‐1. The function of PMNs eluted from filters with or without the aid of manual tapping (MT) at each of the various FLTs was assessedin vitroby tests of chemotaxis, phagocytosis and bactericidal capacity. The results indicated that the function of PMNsin vitrowas compromised by eluting filters with MT, and this appeared to be independent of the FLT. PMNs collected without MT were nearly normal relative to the control regardless of the F