USE of an ELECTRONIC HGMF INTERPRETER SYSTEM FOR ENUMERATION of NALIDIXIC ACID RESISTANT SALMONELLAE IN CHICKEN CAECA

摘要

An electronic counting system using hydrophobic grid‐membrane filters (HGMF) and the HGMF Interpreter was evaluated for its usefulness in enumerating nalidixic acid resistantSalmonellain frozen chicken caeca.Salmonellarecovery was equivalent on both Hektoen Enteric and EF‐18 agars. However, the color of theSalmonellagrowth on EF‐18 agar was more easily differentiated by the HGMF Interpreter electronic counting system. the study showed that a 4 h resuscitation on a nonselective medium was required in order to maximize the subsequent recovery on Hektoen Enteric agar, though not on EF‐18 agar. Using the EF‐18 agar as theSalmonellaselective medium, a method was established that recorded counts of nalidixic acid resistantSalmonellarapidly and easily in electronic data files, for subsequent retrieval and man