Micropropagation of Sauropus androgynus (L.) Merr.-An important green leafy vegetable

摘要

A micropropagation protocol has been developed for Sauropus androgynus (L.) Merr. using uninodal explants in Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinins, benzyl adenine (BA) and kinetin (Kn). Shoot induction was observed in 1.0 mg/L BA and 0.1 mg/L Kn after 25 d and more number of shoots was achieved in shoot induction medium. Rooting was induced from shoots in MS medium supplemented with various concentrations of indole 3-butyric acid (IBA) and naphthalene acetic acid (NAA). All the shoots were rooted in 0.5 mg/L IBA and 0.2 mg/L NAA after 25 d. Rooted plants were transferred to soil and successfully acclimatized.