Homology modeling and enzyme function prediction in uncharacterized proteins of Salmonella typhi - an in silico approach.

摘要

Salmonella Typhi, a known human pathogen registering multiple drug resistance, causes the majority of endemic cases in developing nations. In this study, the Salmonella Typhi genome was marked with 1220 ORFs encoding hypothetical proteins. Enzyme coding probability was evaluated in hypothetical proteins using web tools such as CDD-BLAST, InterProScan, Pfam and COGs. Results identified 213 proteins as enzyme coding, and for these proteins tertiary structures were predicted based on homology modelling. Approx. 89 structures were modelled for functional proteins and it is suggested that these deciphered structure-function relationships could aid understanding of the pathogenicity and stress resistance of Salmonella Typhi.