Micropropagation of Stachytarpheta jamaicensis (L.) Vahl-A medicinally important plant

摘要

An in vitro-protocol for micropropagation of Stachytarpheta jamaicensis (L.) Vahl was developed through nodal explants. Murashige and Skoog (MS) medium with BA 0.5 mg/L and IBA 0.5mg/L was found to be the best for multiple shoot formation from nodal explants. The maximum response of shoot induction was observed on a medium containing 1.0 mg/L Kn. The regenerated shoots were excised and transferred to rooting medium containing IBA at different concentrations. Highest of 84% rooting was observed at 0.5 mg/L level of IBA and BA. The in vitro derived plantlets were hardened and acclimatized in soil. About 75% of plantlets survived in the field condition.