Rapid typing of Pasteurella multocida isolates from haemorrhagic septicaemia cases by multiplex PCR for their virulence

摘要

A total of 12 P. multocida isolates recovered from field outbreaks of haemorrhagic septicaemia in buffaloes were studied for the presence of 4 virulence genes (pfhA, tbpA, hgbB & toxA) in comparison with vaccine strain by multiplex PCR. The study showed the presence of pfhA and tbpA in all the field isolates and vaccine strain. None of the isolates harboured toxA and hgbB. The virulence genes tbPA and pfhA appear to be closely related to bovine P. multocida serogroup B isolates and are important for severe form of the disease.