Effect of Silicone Antifoam on Shear Sensitivity of Hybridoma Cells in Sparged Cultures

摘要

AbstractAddition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a sparged medium with a protective component, such as is applied for large‐scale animal cell culture