Agrobacterium-mediated genetic transformation of indica rice varieties involving Am-SOD gene

摘要

Transgenic plants containing Am-SOD gene developed from embryogenic calli of Pusa Basmati 1, Taraori Basmati and, a new plant type (NPT) super rice variety, IR 70485-15-3-2 enroute co-cultivation with Agrobacterium tumifaciens. Am-SOD gene was housed under transcriptional control of CaMV 35S promoter and Nos polyadenylation signal in binary vector pCAMBIA 1301. Transformation efficiency was found to be very good. Molecular analysis involving PCR, slot and Southern blot showed integration of the gene at genome level in a few plants, which was further substantiated through an array of physiological studies conducted in vitro.