The histological changes in the ovary of sevenband grouper Epinephelus septemfasciatus were examined during vitellogenesis and final oocyte maturation. Ovaries contained oocytes at the perinucleolus stage at the start of the experiment in January. Oocytes at the primary yolk stage appeared when the gonadosomatic index (GSI) was slightly on the rise in March. After that, vitellogenesis progressed and ovaries contained oocytes at the tertiary yolk stage in May. The GSI increased drastically at the sametime. These results suggest that vitellogenesis in sevenband grouper starts or is already in progress in March and is completed by May, In order to clarify the process of final oocyte maturation, luteinizing hormone-releasing hormone (LHRH) analog (desGly~(10), D-Ala~6 LHRH ethylamide) was implanted in fish with oocytes at the tertiary yolk stage.The oocytes developed to the migratory nucleus stage in 18 h and reached the maturation stage in 42 h after implantation. This indicates that final oocytematuration is completed within 42 h after stimulation of gonadotropin-releasing hormone.
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