Influence of some plant growth regulators on the growth and organogenesis of Cymbidium aloifolium (L.) Sw. seed-derived rhizomes in vitro.

摘要

An efficient procedure is outlined for the in vitro regeneration of an epiphytic orchid, C. aloifolium, using rhizomes developed from seeds. Murashige and Skoog's medium (MS) containing IAA, IBA or NAA stimulated growth and proliferation of rhizomes, with NAA being the most effective at 5.0 mg/litre (27.0 鍹). Shoot bud differentiation was induced in the apical portions of the rhizomes on MS medium containing kinetin (Kn) or N6-benzyladenine (BA). The highest frequency of shoot regeneration (91.5%)and the highest number of shoot buds formed (3.5 shoots/rhizome) were recorded with BA at 1.0 mg/litre (4.4 鍹). NAA (0.1 mg/litre, 0.54 鍹), when added to the medium in conjunction with BA (1.0 mg/litre, 4.4 鍹), slightly enhanced the frequency of shoot bud regeneration (92.6%) and the number of shoot buds formed (5.2 shoots/rhizome). Moreover, an NAA-BA combination induced rooting in regenerated shoots thereby producing complete plantlets in one step. Shoots developed on cytokinin-supplemented mediumwere rooted on MS medium containing NAA at 1.0 mg/litre (5.4 鍹). Regenerated plantlets were acclimated and eventually established in a garden.