The 5′ untranslated region ofArabidopsis thalianacalmodulin cDNA is an independent cDNA containing an open reading frame

摘要

A cDNA clone, pAUK1, with an open reading frame (ORF) coding for a hypothetical 164-amino-acid protein was isolated from anArabidopsis thaliana(L.) Heynh cDNA library. The clone was attached, tail to tail, to the 3′ end ofA. thalianahexokinase cDNA. An almost identical sequence had been previously described as the 5′ untranslated region (5′ UTR) ofA. thalianacalmodulin cDNA (ACaM-2). Sequence comparison with three additionalA. thalianatruncated cDNA clones which appear in a database (GenBank) supports the conclusion that pAUKl is identical to the 5′ UTR of ACaM-2 and that the 5′ UTR of ACaM-2 is an independent cDNA artificially linked toA. thalianacalmod