Changes in Enzymatic Activities of Dark CO_2 Metabolism and Sucrose Metabolism during Fruit Development in Brassica sp.

摘要

Enzymes PEP carboxylase, NADP* malate dehydrogenease, NADP* malic enzyme and glutamate oxaloacetate transminase decreased as the stage advanced (i.e. 10 to 40 DAA) in fruit wall as well as developing seeds. B. oxyrrhina had more activity of these enzymes than B. tournefortii. Both the species had maximum activity of Glutamate exalacetate transaminase compared with other enzymes in fruit wall and developing seeds at all the stages. Comparison of enzyme activities of sucrose metabolism revealed that Fructose 1, 6-biphosphatase decreased as the stage advanced in both the species where as revers trend was noticed in case of sucrose phosphate synthase. Fruit wall had three fold more activity of surcorse phosphate synthase than in seeds whereas activity of F1, 6 BP was higher in developing seeds than fruit wall in both the species.