Separation of cells with different histocompatibility (H‐2) antigens by passage through cellular immunoadsorbent columns

摘要

AbstractThe anti‐Ig column technique of Wigzell et al.(Scand. J. Immunol.1972.1: 75) was adapted to the separation of mouse cells with different H‐2 antigens. Fractionation was achieved by a two‐step procedure: first the cells were treated with anti‐H‐2 sera, and thereafter passed over anti‐mouse Ig columns. Antibody‐coated cells were selectively retained in the anti‐Ig column. The method could be applied to the separation of normal mouse lymphocytes or lymphoma cells with different H‐2 antigen complexes and for the selective isolation of H‐2 loss variants from a mouse carcinom