Isolation and Characterization of Antibiotic Producing Actinomycetes from Soil and Effect of Various Physical Parameters on Antibiotic Production

摘要

Total twenty three isolates of actinomyces were recovered from the soil of different region of IVIohali and Bathinda region and screened for their antibacterial activity against test organisms Kleibsella, Bacillus, Pseudomonas and Staphylococcus. Fourisolates were recorded to show antibiotic production, out of which two isolates named KA1 and KA2 were found most effective. The isolate KA2 showed maximum antibiotic production at neutral pH with 1% NaCI concentration at 30°C after 72 hours of incubation. Various biochemical and morphological tests along with some special tests were performed with KA1 and KA2 and results were confirmed with Bergay's manual for their belonging to sub-family streptomyces.