Effect of supplementation of glutamic acid and aspartic acid in egg yolk tris and egg yolk-citrate-glucose diluents on cryopreservation of Murrah but talcbull spermatozoa

摘要

Present study was designed to assess the effect of supplementation of glutamic acid and aspartic acid in egg yolk tris and egg yolk-citrate-glucose diluents on spermatozoa quality of Murrah buffalo bull after equilibration and thawing. Semen was collected from four Murrah buffalo bulls biweekly from each bull with the help of artificial vagina (AV) using standard protocol. A total of thirty two ejaculates were collected from four bulls. The fresh ejaculates were pooled and divided into eight equal parts. The two basic diluents used for extending semen were Egg yolk Tris (EYT) and Egg yolk-citrate glucose (EYCG). Glutamic acid (4 mM) and aspartic acid (0.40 mM) were supplemented in different combinations. Diluted semen was equilibrated and cryopreserved. The progressive motility, live and dead spermatozoa and acrosomal damage was assessed after equilibration and thawing. Post thaw acrosomal damage was significantly higher (F<0.05) in EYCG than EYT. Supplementation of aspartic acid to EYT and EYCG significantly (PO.05) decreased the post thaw acrosomal damage as compared to control. Supplementation of glutamic acid to EYT and EYCG significantly (PO.05) improved all the studied spermatozoa qualities. The addition of both aspartic and glutamic acid together did not produce any beneficial effect on spermatozoa quality. It can be concluded that tris buffer was a better substitute to citrate buffer and glutamic acid alone may be supplemented to semen extender to improve the spermatozoa quality after equilibration and thawing.