Effect of Different Supplementation in The Maturation Media (TCM-199) on In-Vitro Maturation of Prepubertal and Pubertal Goat Oocytes

摘要

The study was carried out with the aim to study the efficacy of different supplementation on iivvitro maturation of goat oocytes. A total of 686 and 733 numbers of good quality (Grade A and B) cumulus oocytes complexes (COCs), were collected by puncturing surface follicle (non atretic) of 332 prepubertal and 686 pubertal ovaries. These were put for maturation in maturation media (TCM-199) containing either 20 % estrous goat serum (EGS), 20 % new calf serum (NCS), 70 % new calf serum (NCS) + Hormone (FSH + LH + E2) or 20 % caprine follicular fluid (OFF). Maturation was assessed 27 h (hours) later from the oocytes that had been placed in a culture (in a CO_2 incubator in humidified air at 38.5 1degC with 5 % CO_2). On morphological basis the maturation rate (%) of oocytes in using 20 % estrous goat serum (EGS), 20 % new calf serum (NCS), W % new calf serum plus hormone (FSH + LH + E_2) or 20 % caprine follicular fluid (CFF) for prepubertal and pubertal animals were 86.98, 88.35, 94.31, 88.99 and 89.75, 88.023, 92.48, 89.56 per cent respectively.