The Determination of Urinary Metanephrine and Normetanephrine by High Performance Liquid Chromatography with Ultraviolet Absorbance Detection

摘要

The sensitive and specific direct analysis of urinary normetanephrine and metanephrine (both free and conjugated) was achieved utilizing reversed-phase high performance liquid chromatography and UV detection. The urine specimens were hydrolysed in a boiling water bath and treated with isoamyl alcohol:toluene to eliminate interference from urinary pigments or related chemical compounds. The methoxyamines were isolated by the passage through a small ion-exchange column and then pre-concentrated by a rapid set of solvent (ethyl acetate:acetone) extractions before being injected into the chromatographic column.