We present a spectioscopic analysis of Red Blood Cells (RBCs), The analysis was canied out at the level of single cells, which were optically tiapped by means of a near-infrared laser (A = 1064 nm). Resonance Raman excitation of hemoglobin (Hb) was per formed with a second laser, emitting at A = 532 nm,. The aim of this study is to compare the Raman response of normal RBCs with tha-lassemic cells. Raman analysis is used to draw hemoglobin distribution inside single erythrocytes In particular, the experimental outcomes reveal the characteristic anomaly (target shape) occurring in thalassemia These results in vivo show the potential of our technique as a diagnostic tool for other hemopathies.
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