Structural determination of subsidiary colors in commercial Food Green No. 3 (Fast green FCF, FD C Green No. 3)

摘要

HPLC analysis revealed that eight subsidiary colors existed in commercial Food Green No. 3 k(fast green FCF, FD C Green No. 3). Among them, four subsidiary colors C, F, G, and H were isolated by using preparative HPLC and their structures were determined by MS and NMR. They were the disodium salt of 2-4-N-ethyl-N-(3-sulfophenylmethyl)aminophenyl)4-N-ethyl-N-(4-sulfop henylmethyl)aminophenylmethylio-4-hydroxybenzenesulfonic acid (abbreviated as m,p-G-3), the sodium salt of 2-(4-N-ethylamino)phenyl4-N-ethyl-N-(3-sulfophenylmetliyl)aminopheny lmethylio-4-hydroxybenzenesulfonic acid abbreviated as HSBA-(EA) (m-EBASA), the sodium salt of 2-(4-N-diethylamino)phenyl4-N-ethyl-N-(3-sulfophenylmethyl)aminophen ylmethylio-4-hydroxybenzenesulfonic acid abbreviated as HSBA-(di-EA) (m-EBASA), and the sodium salt of 2-4-N-ethyl-N-(phenylmethyl)aminophenyl4-N-ethyl-N-(3-sulfophenylme tliyl)amino phenyl methylio-4-hydroxybenzenesulfonic acid abbreviated as HSBA-(EBA) (m-EBASA), respectively. HSBA-(di-EA) (m-EBASA) was a subsidiary color newly found in commercial Food Green No. 3.