Perillacell suspension cultures contained caffeic acid at 0.073of the fresh weight. When 1 min glyphosate was administered to the cell culture in the logarithmic and stationary phases, the amount of caffeic acid ceased to increase and remained at a nearly constant level during the following several days. Phenylalanine ammonia-lyase (PAL) activity in the cell culture increased immediately after transfer to a fresh medium, decreased rapidly in the midst of the logarithmic phase and became almost undetectable in the stationary phase. PAL activity was markedly inhibited by glyphosate at more than 0.2 mM. The shikimic acid content of cells from 14-day culture grown in the presence of 1 mM glyphosate increased up to 74.9μg per g fresh weight during 6-day culture, whereas that of the control cells was undetectable. The dosage of 0.15 mM L-2-aminooxy-3-phenylpropionic acid (l-AOPP), an inhibitor of PAL, to the cells did not cause shikimic acid accumulation
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